2XZI
THE ASPERGILLUS FUMIGATUS SIALIDASE IS A KDNASE: STRUCTURAL AND MECHANISTIC INSIGHTS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.810, 57.990, 94.830 |
| Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
| Resolution | 37.310 - 1.450 |
| R-factor | 0.18045 |
| Rwork | 0.179 |
| R-free | 0.20459 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.286 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.080 | 0.240 |
| Number of reflections | 143277 | |
| <I/σ(I)> | 9.4 | 4.3 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
