2XZA
Crystal Structure of recombinant A.17 antibody FAB fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-19 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.150, 66.710, 66.100 |
| Unit cell angles | 90.00, 107.20, 90.00 |
Refinement procedure
| Resolution | 7.990 - 1.500 |
| R-factor | 0.16759 |
| Rwork | 0.166 |
| R-free | 0.21095 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.378 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.010 | 0.050 |
| Number of reflections | 68859 | |
| <I/σ(I)> | 23.8 | 3.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M ADA BUFFER PH 6.5 0.1M MGCL2 20% PEG6000 |
