2XZ1
The Structure of the 2:2 (Fully Occupied) Complex Between Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean) and Acyl Carrier Protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 76.404, 76.404, 403.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.350 |
| R-factor | 0.21155 |
| Rwork | 0.209 |
| R-free | 0.25451 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.094 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.440 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 16101 | |
| <I/σ(I)> | 9.7 | 2.7 |
| Completeness [%] | 92.7 | 96 |
| Redundancy | 2.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 16-20% MPD,6-8% PEG20000, 0.1M SODIUM CACODYLATE PH 5.8, 4-6% ACETONITRILE |
