2XYM
HCV-JFH1 NS5B T385A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.580, 110.810, 115.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.822 - 1.774 |
| R-factor | 0.1813 |
| Rwork | 0.179 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i5k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.001 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.822 | 1.880 |
| High resolution limit [Å] | 1.774 | 1.770 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 60230 | |
| <I/σ(I)> | 14.1 | 2.8 |
| Completeness [%] | 99.1 | 95.7 |
| Redundancy | 5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NAH2PO4, 2-5% PEG 35.000 |
