2XYB
CRYSTAL STRUCTURE OF A FULLY FUNCTIONAL LACCASE FROM THE LIGNINOLYTIC FUNGUS PYCNOPORUS CINNABARINUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-13 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.296, 62.902, 91.416 |
| Unit cell angles | 90.00, 126.70, 90.00 |
Refinement procedure
| Resolution | 72.550 - 1.750 |
| R-factor | 0.16639 |
| Rwork | 0.164 |
| R-free | 0.20814 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gyc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.778 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.040 | 0.440 |
| Number of reflections | 66427 | |
| <I/σ(I)> | 27.4 | 1.3 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 12.0 MG/ML PROTEIN, 14 % PEG 8000, 20 % GLYCEROL, 160 MM ZN-ACETATE, 5 MM CU-SULAFTE, 100 MM NA-CACODYLATE, PH 5.9 |
