2XXY
Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.665, 99.442, 124.627 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.846 - 3.000 |
| R-factor | 0.2242 |
| Rwork | 0.222 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xxt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.180 | 0.660 |
| Number of reflections | 21368 | |
| <I/σ(I)> | 14.2 | 3.5 |
| Completeness [%] | 97.5 | 98.7 |
| Redundancy | 7.6 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP 21% PEG 4000, 3% PROPAN-2-OL, 80MM SODIUM ACETATE |
