2XXR
Crystal structure of the GluK2 (GluR6) wild-type LBD dimer in complex with glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 95.326, 105.464, 114.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.439 - 1.600 |
| R-factor | 0.1706 |
| Rwork | 0.169 |
| R-free | 0.19480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s50 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.016 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.660 |
| Number of reflections | 75578 | |
| <I/σ(I)> | 18.9 | 2.3 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 5.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP. 27% PEG 4000, 3% PROPAN-2-OL, 80MM SODIUM ACETATE |
