2XXQ
A widespread family of bacterial cell wall assembly proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-09-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.500, 73.500, 163.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.640 - 1.770 |
| R-factor | 0.17152 |
| Rwork | 0.170 |
| R-free | 0.19893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xxp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.270 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.880 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 44731 | |
| <I/σ(I)> | 21.6 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.8 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M SODIUM ACETATE PH 4.6, 8 % PEG W/V 4000 |
