2XXN

Structure of the vIRF4-HAUSP TRAF domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 32 2 1
Unit cell lengths	72.462, 72.462, 53.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.600
R-factor	0.15861
Rwork	0.158
R-free	0.17426
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f1w
RMSD bond length	0.008
RMSD bond angle	1.131
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.060	0.270
Number of reflections	21908
<I/σ(I)>	65.2	11.9
Completeness [%]	100.0	100
Redundancy	21.7	21.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.9		5% PEG3350, 0.2 M MAGNESIUM FORMATE, PH 5.9