2XXK
SiaP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.700, 74.740, 87.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.160 - 1.480 |
| R-factor | 0.10809 |
| Rwork | 0.106 |
| R-free | 0.15334 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4c |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.881 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (RIGID BODY) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.620 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.060 | 0.160 |
| Number of reflections | 52602 | |
| <I/σ(I)> | 22.7 | 9.9 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 35 MG/ML SIAP (IN 20 MM HEPES, 10 MM NACL, 10 MM NEU5AC, PH 8.0) AND EQUAL VOLUME OF RESERVOIR SOLUTION (100MM MES PH 6.0 28.5% PEG 6K, 5% JEFFAMINE M600 R, PH7 (V/V)) TEMPERATURE 277K |
