2XX6

Structure of the Bacillus subtilis prophage dUTPase, YosS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-25
Detector	ADSC QUANTUM 315r
Spacegroup name	P 63
Unit cell lengths	102.763, 102.763, 86.174
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.740
R-factor	0.17372
Rwork	0.171
R-free	0.21857
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dut
RMSD bond length	0.024
RMSD bond angle	1.910
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.990	1.800
High resolution limit [Å]	1.740	1.760
Rmerge	0.060	0.450
Number of reflections	52906
<I/σ(I)>	24.5	2.3
Completeness [%]	99.5	94
Redundancy	5.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		0.1M IMIDAZOLEMALATE BUFFER PH 5.5, 30% (V/V) PEG600