2XX6
Structure of the Bacillus subtilis prophage dUTPase, YosS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 63 |
| Unit cell lengths | 102.763, 102.763, 86.174 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.740 |
| R-factor | 0.17372 |
| Rwork | 0.171 |
| R-free | 0.21857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dut |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.910 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.990 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.760 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 52906 | |
| <I/σ(I)> | 24.5 | 2.3 |
| Completeness [%] | 99.5 | 94 |
| Redundancy | 5.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M IMIDAZOLEMALATE BUFFER PH 5.5, 30% (V/V) PEG600 |
