2XVO

SSO1725, a protein involved in the CRISPR/Cas pathway

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	77.750, 90.290, 111.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.480 - 2.080
R-factor	0.20066
Rwork	0.199
R-free	0.23051
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.311
Data reduction software	xia2
Data scaling software	xia2
Refinement software	REFMAC (5.6.0081)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.480	2.130
High resolution limit [Å]	2.080	2.080
Rmerge	0.040	0.570
Number of reflections	46758
<I/σ(I)>	19.2	2.2
Completeness [%]	97.8	86.1
Redundancy	4.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1