2XVG

crystal structure of alpha-xylosidase (GH31) from Cellvibrio japonicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-26
Detector	ADSC CCD
Spacegroup name	P 63 2 2
Unit cell lengths	156.193, 156.193, 227.759
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.885 - 2.600
R-factor	0.2065
Rwork	0.204
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g3m
RMSD bond length	0.009
RMSD bond angle	1.156
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.900	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.110	0.810
Number of reflections	50969
<I/σ(I)>	14.4	2.7
Completeness [%]	99.9	100
Redundancy	11	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		25 % PEG MONOMETHYL ETHER 550 (PEG MME 550), 0.1 M BIS-TRIS (PH 7.0)