2XV2
Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM), chemically reduced, pH4.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.897, 46.249, 57.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.130 - 1.600 |
| R-factor | 0.14325 |
| Rwork | 0.140 |
| R-free | 0.20039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fs9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.679 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.210 |
| Number of reflections | 14745 | |
| <I/σ(I)> | 25.6 | 7 |
| Completeness [%] | 99.7 | 97.7 |
| Redundancy | 6.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1M POTASSIUM THIOCYANATE, 30% PEG2000, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
