2XUL
Structure of PII from Synechococcus elongatus in complex with 2- oxoglutarate at high 2-OG concentrations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.870, 87.987, 116.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.090 - 2.200 |
R-factor | 0.18161 |
Rwork | 0.179 |
R-free | 0.22488 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.029 |
RMSD bond angle | 2.041 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.120 | 0.780 |
Number of reflections | 36239 | |
<I/σ(I)> | 2.6 | 2.6 |
Completeness [%] | 99.6 | 98.1 |
Redundancy | 7.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | pH 7.4 |