2XUB
Human RPC62 subunit structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.790, 69.380, 75.400 |
| Unit cell angles | 90.00, 113.20, 90.00 |
Refinement procedure
| Resolution | 43.130 - 2.800 |
| R-factor | 0.1905 |
| Rwork | 0.188 |
| R-free | 0.22980 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | BUSTER (2.9.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 19023 | |
| <I/σ(I)> | 14.1 | 2 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 5.26 | 5.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
