2XTQ
Structure of the P107A Colicin M mutant from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.852, 63.114, 189.340 |
| Unit cell angles | 87.59, 82.17, 65.65 |
Refinement procedure
| Resolution | 48.680 - 2.310 |
| R-factor | 0.24185 |
| Rwork | 0.240 |
| R-free | 0.27261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmx |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.370 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 85754 | |
| <I/σ(I)> | 5.4 | 1.5 |
| Completeness [%] | 89.5 | 72.9 |
| Redundancy | 1.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG3350 0.2 M NANO3, pH 6.5 |
