2XT2
Structure of the pentapeptide repeat protein AlbG, a resistance factor for the topoisomerase poison albicidin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU-MSC RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.710, 90.242, 55.983 |
| Unit cell angles | 90.00, 100.94, 90.00 |
Refinement procedure
| Resolution | 22.560 - 1.999 |
| R-factor | 0.1661 |
| Rwork | 0.163 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.204 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.030 | 0.110 |
| Number of reflections | 29136 | |
| <I/σ(I)> | 24.8 | 8.7 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | COMBINING NATIVE PROTEIN, 2 UL OF 20 MG ML-1, 20 MM TRIS PH 8.0, 1 MM DTT WITH PRECIPITANT, 2 UL OF 1 M MGSO4, 100 MM LISO4, 100 MM ADA PH 6.3 |
