2XT1
Crystal structure of the HIV-1 capsid protein C-terminal domain (146- 231) in complex with a camelid VHH.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.060, 88.170, 35.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.540 - 1.320 |
| R-factor | 0.1618 |
| Rwork | 0.161 |
| R-free | 0.18350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2BUO 1kxv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 1.390 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 45032 | |
| <I/σ(I)> | 14.7 | 2.1 |
| Completeness [%] | 98.2 | 88.6 |
| Redundancy | 13.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 28-32 % W/V PEG 4000 |
