2XSV
Crystal structure of L69A mutant Acinetobacter radioresistens catechol 1,2 dioxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.646, 78.905, 76.181 |
| Unit cell angles | 90.00, 126.07, 90.00 |
Refinement procedure
| Resolution | 41.070 - 1.800 |
| R-factor | 0.17559 |
| Rwork | 0.175 |
| R-free | 0.19886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2azq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.744 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.070 |
| Number of reflections | 44716 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 99.3 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
