2XST
Crystal Structure of the Human Lipocalin 15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 44.800, 44.800, 126.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.800 - 1.630 |
| R-factor | 0.1576 |
| Rwork | 0.156 |
| R-free | 0.18520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 1.720 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.080 | 0.820 |
| Number of reflections | 17872 | |
| <I/σ(I)> | 11.3 | 2 |
| Completeness [%] | 99.8 | 1.6 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH7.5, 1.4 M TRI-SODIUM CITRATE DIHYDRATE |
