2XSN
Crystal Structure of Human Tyrosine Hydroxylase Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 191.420, 191.420, 168.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.660 - 2.680 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.120 | 2.820 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.060 | 1.240 |
| Number of reflections | 51392 | |
| <I/σ(I)> | 11.2 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM ACETATE, 0.1 M TRI-SODIUM CITRATE, 30% PEG 4K. |
