2XSM
Crystal structure of the mammalian cytosolic chaperonin CCT in complex with tubulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 272.700, 313.500, 158.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 5.500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0099) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 6.000 |
| High resolution limit [Å] | 5.500 | 5.500 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 74325 | |
| <I/σ(I)> | 9.54 | 1.2 |
| Completeness [%] | 92.5 | 95.3 |
| Redundancy | 4.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1 M LICL, 100 MM MES PH 6, 10% PEG6000, 10% OF THE OPTIC SALT SUITE NUMBER 63 FROM QIAGEN, AND 5% OF TERT-BUTANOL FROM HAMPTON RESEARCH ADDITIVE SCREEN. |
