2XSL
The crystal structure of a Thermus thermophilus tRNAGly acceptor stem microhelix at 1.6 Angstroem resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.187, 29.015, 29.534 |
| Unit cell angles | 107.17, 97.37, 96.55 |
Refinement procedure
| Resolution | 27.800 - 1.590 |
| R-factor | 0.23898 |
| Rwork | 0.237 |
| R-free | 0.28033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ARTIFICIAL RNA |
| RMSD bond length | 0.025 |
| RMSD bond angle | 3.138 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 10142 | |
| <I/σ(I)> | 14.5 | 1.7 |
| Completeness [%] | 94.7 | 72.9 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.5 MM RNA IN 40 MM SODIUM CACODYLATE, PH 7.0, 12 MM SPERMINE TETRA-HCL, 80 MM NACL AND 10 % (V/V) MPD, EQUILIBRATED AGAINST 35 % (V/V) MPD, ROOM TEMPERATURE. |
