2XSE
The structural basis for recognition of J-base containing DNA by a novel DNA-binding domain in JBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2010-03-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 67.338, 67.338, 186.762 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.320 - 1.900 |
| R-factor | 0.17553 |
| Rwork | 0.174 |
| R-free | 0.20038 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.255 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX.HYSS |
| Refinement software | REFMAC (5.6.0081) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.660 |
| Number of reflections | 20683 | |
| <I/σ(I)> | 10.9 | 2.3 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 5.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20MM HEPES PH 7.5, 50MM NACL, 1MM TCEP 15.3% PEG 6000, 0.2M POTASSIUM NITRATE, 20% GLYCEROL (CRYO-PROTECTANT) |
