2XSD
Crystal Structure of the dimeric Oct-6 (Pou3f1) POU domain bound to palindromic MORE DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-19 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.497, 52.009, 69.013 |
| Unit cell angles | 90.00, 129.03, 90.00 |
Refinement procedure
| Resolution | 41.389 - 2.049 |
| R-factor | 0.1978 |
| Rwork | 0.194 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e3o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.153 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.040 | 0.450 |
| Number of reflections | 15965 | |
| <I/σ(I)> | 31.7 | 1.9 |
| Completeness [%] | 98.1 | 89.2 |
| Redundancy | 7.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | RESERVOIR: 0.2M MGCL2, 14%PEG4000 AND 0.1 SODIUM CITRATE PH5.6 PROTEIN-DNA COMPLEX: 10MG/ML IN 10 MM HEPES PH 7.0; 100 MM NACL EUQAL RESERVOIR AND PROTEIN VOLUMES MIXED |
