2XS2
Crystal structure of the RRM domain of mouse Deleted in azoospermia-like in complex with RNA, UUGUUCUU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-14 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.100, 52.060, 60.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.410 - 1.350 |
| R-factor | 0.16292 |
| Rwork | 0.161 |
| R-free | 0.19267 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.642 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.410 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.060 | 0.650 |
| Number of reflections | 26034 | |
| <I/σ(I)> | 15 | 2.4 |
| Completeness [%] | 96.0 | 87.3 |
| Redundancy | 5.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 MM MAGNESIUM FORMATE, 20 % (W/V) PEG 3350, 0.1 MM ZINC ACETATE |
