2XRO

Crystal structure of TtgV in complex with its DNA operator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 65
Unit cell lengths	89.584, 89.584, 416.747
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.225 - 3.400
R-factor	0.217
Rwork	0.214
R-free	0.28230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xrn
RMSD bond length	0.010
RMSD bond angle	1.238
Data reduction software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	77.500
High resolution limit [Å]	3.400
Rmerge	0.060
Number of reflections	25878
Completeness [%]	96.7
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1