2XRM
Processed Intracellular subtilisin from B. clausii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 132.982, 132.982, 132.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.490 - 2.600 |
| R-factor | 0.20175 |
| Rwork | 0.198 |
| R-free | 0.28084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x8j |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.824 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0081) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.400 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 1.410 |
| Number of reflections | 12244 | |
| <I/σ(I)> | 13.4 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.6 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | pH 6.7 |
