2XRI
Crystal structure of human ERI1 exoribonuclease 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.720, 76.900, 86.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.490 - 2.150 |
| R-factor | 0.17674 |
| Rwork | 0.175 |
| R-free | 0.21014 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0h |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 12879 | |
| <I/σ(I)> | 22.1 | 6.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.6 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH 8.5 20% PEG MONOMETHYL ETHER 2000 0.2M TRIMETHYLAMINE N-OXIDE |
