2XRD
Structure of the N-terminal four domains of the complement regulator Rat Crry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 205.840, 100.370, 21.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.090 - 3.500 |
| R-factor | 0.2498 |
| Rwork | 0.250 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1OK9 AND 1GKN |
| Data reduction software | XIA (USING XDS) |
| Data scaling software | XIA (USING SCALA) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.230 | 0.480 |
| Number of reflections | 7742 | |
| <I/σ(I)> | 8 | 2.6 |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 5.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M SODIUM ACETATE PH 4.6, 2.0 M SODIUM CHLORIDE. |
