2XQX
Structure of the family 32 carbohydrate-binding module from Streptococcus pneumoniae EndoD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.105, 73.510, 34.597 |
| Unit cell angles | 90.00, 96.52, 90.00 |
Refinement procedure
| Resolution | 66.670 - 2.010 |
| R-factor | 0.21782 |
| Rwork | 0.215 |
| R-free | 0.26216 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.256 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 0.310 |
| Number of reflections | 20817 | |
| <I/σ(I)> | 18.5 | 7.2 |
| Completeness [%] | 98.1 | 97.4 |
| Redundancy | 14.6 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
