2XQQ
Human dynein light chain (DYNLL2) in complex with an in vitro evolved peptide (Ac-SRGTQTE).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-03 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.600, 64.000, 151.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 151.840 - 1.310 |
| R-factor | 0.12447 |
| Rwork | 0.123 |
| R-free | 0.15636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cmi |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.010 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 151.840 | 1.340 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 81872 | |
| <I/σ(I)> | 15.66 | 2.93 |
| Completeness [%] | 96.0 | 67.1 |
| Redundancy | 7.6 | 3.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | HANGING DROP VAPOR DIFFUSION AT 293K WITH A RESERVOIR SOLUTION 31% PEG 4000, 0.4 M NH4AC, 0.1 M NAAC PH 4.6, 2UL RESERVOIR SOLUTION AND 2UL PROTEIN SOLUTION |
