2XQB
Crystal Structure of anti-IL-15 Antibody in Complex with human IL-15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-26 |
| Detector | RIGAKU CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.186, 43.754, 70.087 |
| Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
| Resolution | 69.710 - 2.600 |
| R-factor | 0.24809 |
| Rwork | 0.244 |
| R-free | 0.31811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.605 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.700 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.130 | 0.360 |
| Number of reflections | 17425 | |
| <I/σ(I)> | 4.4 | 2 |
| Completeness [%] | 99.1 | 98.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | PCTP 100MM, PH 9.5, 25 %W/V PEG-3350, 200MM AMMONIUM SULPHATE |
