2XQ6
Pentameric ligand gated ion channel GLIC in complex with cesium ion (Cs+)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2009-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.314, 128.310, 164.374 |
| Unit cell angles | 90.00, 104.04, 90.00 |
Refinement procedure
| Resolution | 39.866 - 3.700 |
| R-factor | 0.2466 |
| Rwork | 0.246 |
| R-free | 0.25780 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.412 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.700 |
| Rmerge | 0.140 |
| Number of reflections | 40144 |
| <I/σ(I)> | 8.9 |
| Completeness [%] | 88.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 9% PEG4000, 50MM CH3COONA, 225MM (NH4)2SO4, PH4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277.15K, VAPOR DIFFUSION, HANGING DROP |
