2XPY

Structure of Native Leukotriene A4 Hydrolase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	159.556, 159.556, 76.832
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.005 - 2.730
R-factor	0.1779
Rwork	0.175
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hs6
RMSD bond length	0.008
RMSD bond angle	1.148
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.090	0.470
Number of reflections	30000
<I/σ(I)>	7.4	1.6
Completeness [%]	95.3	68.8
Redundancy	4.1	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			TO OBTAIN DIFFRACTION QUALITY CRYSTALS, HANGING DROPS OF 2 UL OF PROTEIN (6 MG/ML) AND 1 UL OF MOTHER LIQUOR (0.1 M MMT BUFFER, PH 8, 12-14% PEG 6000) WERE ALLOWED TO EQUILIBRATE AGAINST THE MOTHER LIQUOR FOR 18-24 HOURS BEFORE THE DROPS WERE STREAK SEEDED FROM EARLIER CRYSTALS.