2XPP
Crystal structure of a Spt6-Iws1(Spn1) complex from Encephalitozoon cuniculi, Form III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.356, 56.053, 116.444 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.220 - 1.740 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.23381 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xpl |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.621 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.040 | 0.170 |
| Number of reflections | 18952 | |
| <I/σ(I)> | 44.6 | 9.2 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M SUCCINIC ACID/SODIUM DIHYDROGEN PHOSPHATE/GLYCINE SYSTEM PH 7.0 33% PEG 1500 |
