2XPJ
Crystal structure of Physalis Mottle Virus with intact ordered RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 285.400, 288.360, 290.610 |
| Unit cell angles | 62.92, 65.30, 60.85 |
Refinement procedure
| Resolution | 30.000 - 3.400 |
| R-factor | 0.308 |
| Rwork | 0.308 |
| R-free | 0.31120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1auy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.545 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.150 | 0.370 |
| Number of reflections | 767743 | |
| <I/σ(I)> | 6.7 | 2.04 |
| Completeness [%] | 80.0 | 80 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.5M SODIUM ACETATE, 10 MM DITHIOTHRETOL, 2 MM CALCIUM CHLORIDE, 3 % PEG 6000, pH 5.6 |
