2XOZ
C-terminal cysteine rich domain of human CHFR bound to AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.810, 51.560, 82.490 |
| Unit cell angles | 90.00, 105.93, 90.00 |
Refinement procedure
| Resolution | 39.687 - 2.374 |
| R-factor | 0.1838 |
| Rwork | 0.179 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xoc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.043 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.410 | 2.500 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.120 | 0.410 |
| Number of reflections | 26821 | |
| <I/σ(I)> | 8.2 | 3.1 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG 20000, 0.1M MES PH 6.5, 0.1M KCL |
