2XOL
High resolution structure of TtrD from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.763, 52.537, 64.947 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 64.930 - 1.350 |
| R-factor | 0.15248 |
| Rwork | 0.151 |
| R-free | 0.17751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2idg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.277 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.690 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.050 | 0.490 |
| Number of reflections | 89700 | |
| <I/σ(I)> | 17.6 | 4.2 |
| Completeness [%] | 96.9 | 94.9 |
| Redundancy | 6.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M SODIUM FORMATE, 20% PEG 3350, pH 7.5 |
