2XO4
RIBONUCLEOTIDE REDUCTASE Y730NH2Y MODIFIED R1 SUBUNIT OF E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-14 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 224.592, 224.592, 336.719 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 169.031 - 2.500 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.210 | 2.520 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.640 |
| Number of reflections | 112261 | |
| <I/σ(I)> | 10.1 | 1.94 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.47 | 4.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6. |
