2XNR
Structural insights into cis element recognition of non- polyadenylated RNAs by the Nab3-RRM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 |
| Unit cell lengths | 30.640, 30.640, 82.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.640 - 1.600 |
| R-factor | 0.1615 |
| Rwork | 0.160 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xnq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.430 |
| Number of reflections | 9510 | |
| <I/σ(I)> | 14.91 | 5.26 |
| Completeness [%] | 98.9 | 96.3 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 85MM SODIUM ACETATE BUFFER PH 4.6, 170MM AMMONIUM ACETATE, 25.5% (W/V) PEG 4000, 15% (V/V) GLYCEROL |
