2XN1
Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.920, 150.680, 126.680 |
| Unit cell angles | 90.00, 94.29, 90.00 |
Refinement procedure
| Resolution | 21.983 - 2.300 |
| R-factor | 0.1472 |
| Rwork | 0.147 |
| R-free | 0.18710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xn0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.446 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.120 |
| Number of reflections | 181263 | |
| <I/σ(I)> | 24.6 | 12.38 |
| Completeness [%] | 98.5 | 92.4 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 8-12% PEG8000, 0.1M TRIS, 10% GLYCEROL, pH 7.5 |
