2XMM
Visualising the Metal-binding Versatility of Copper Trafficking Sites: H61Y Atx1 side-to-side
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.630, 41.400, 55.580 |
| Unit cell angles | 90.00, 106.76, 90.00 |
Refinement procedure
| Resolution | 53.220 - 1.650 |
| R-factor | 0.16718 |
| Rwork | 0.165 |
| R-free | 0.20037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.225 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.060 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.110 | 0.290 |
| Number of reflections | 16218 | |
| <I/σ(I)> | 14.1 | 4 |
| Completeness [%] | 98.0 | 95.7 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25 % (W/V) PEG 8000, 200 MM SODIUM ACETATE AND 100 MM SODIUM CACODYLATE PH 7.5 |
