2XLV
Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous R124F variant with bound NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.738, 53.738, 180.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.520 - 1.240 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 1.300 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.040 | 0.265 |
| Number of reflections | 41426 | |
| <I/σ(I)> | 35.8 | 4 |
| Completeness [%] | 92.2 | 92.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, TRIS PH 7.5 |
