2XLQ
Structural and Mechanistic Analysis of the Magnesium-Independent Aromatic Prenyltransferase CloQ from the Clorobiocin Biosynthetic Pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-09 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 135.192, 135.192, 98.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.780 - 2.220 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.538 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0091) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.860 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 22556 | |
| <I/σ(I)> | 8.63 | 2.57 |
| Completeness [%] | 97.3 | 81.6 |
| Redundancy | 7.75 | 7.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | VAPOR DIFFUSION. PROTEIN (10 MG/ML) MIXED WITH AN EQUAL VOLUME OF 3 M SODIUM FORMATE, 2 MM DITHIOTHREITOL, 100 MM HEPES PH (6.5). |
