2XLM
Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous recombinant native with bound NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.831, 53.831, 180.703 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.630 - 1.190 |
| R-factor | 0.18768 |
| Rwork | 0.187 |
| R-free | 0.20025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.958 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.900 | 1.230 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.070 | 0.300 |
| Number of reflections | 48344 | |
| <I/σ(I)> | 19.4 | 3 |
| Completeness [%] | 94.7 | 71.3 |
| Redundancy | 6.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, HEPES PH 7.5 |
