2XLJ

Crystal structure of the Csy4-crRNA complex, hexagonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-02
Detector	ADSC CCD
Spacegroup name	P 61
Unit cell lengths	39.250, 39.250, 297.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.625 - 2.600
R-factor	0.2497
Rwork	0.248
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xli
RMSD bond length	0.003
RMSD bond angle	0.653
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.600	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.030	0.310
Number of reflections	7798
<I/σ(I)>	29.8	3.8
Completeness [%]	99.4	98.5
Redundancy	6.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		150 MM SODIUM ACETATE PH 4.6, 17% (W/V) PEG 4000