2XLI
Crystal structure of the Csy4-crRNA complex, monoclinic form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.370, 46.770, 86.820 |
| Unit cell angles | 90.00, 108.62, 90.00 |
Refinement procedure
| Resolution | 19.681 - 2.330 |
| R-factor | 0.2198 |
| Rwork | 0.218 |
| R-free | 0.24690 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.806 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 2.500 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 9974 | |
| <I/σ(I)> | 18.9 | 3.35 |
| Completeness [%] | 96.6 | 98.3 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 200 MM SODIUM CITRATE PH 5.0, 100 MM MAGNESIUM CHLORIDE, 20% (W/V) PEG 4000 |
