2XLB
Acetyl xylan esterase from Bacillus pumilus without ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 143.477, 87.301, 184.291 |
| Unit cell angles | 90.00, 112.89, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.13719 |
| Rwork | 0.136 |
| R-free | 0.15033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ods |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.338 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.250 |
| Number of reflections | 304261 | |
| <I/σ(I)> | 6 | 2 |
| Completeness [%] | 92.1 | 85.2 |
| Redundancy | 4.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M MGCL2, 0.1 M NA HEPES PH 7.5 AND 30 % PEG 400 |
